Could Not Access The SSO Database During Biztalk Runtime Configuration
Solution 1:
How were your service accounts configured in the first environment? Typically a single DEV environment with everything on one box can be done by using a local account on that server. If you now split out your SSO onto another server (it doesn't matter if it's W2K12 instead of W2K8), you are going to have to switch your service account(s) and groups for BizTalk to Domain accounts.
In a multicomputer environment, BizTalk Server supports only domain groups and domain service accounts. Domain groups include Domain Local groups (not recommended), Global groups, and Universal groups. Built-in accounts such as NT AUTHORITY\LOCAL SERVICE, NT AUTHORITY\NETWORK SERVICE, NT AUTHORITY\SERVICE, NT AUTHORITY\SYSTEM, and Everyone are not supported if you want to configure BTS in a multicomputer environment.
Make sure your SSO is running as a domain account, and a member of an SSO Administrators domain group - and ensure this domain account/group combo is configured for the SSO system on the SQL server (instead of local accounts):

After that the SSO system you join from the BizTalk Server before configuring the runtime on BizTalk Server usually needs to be configured with the same domain service account for SSO:

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